In Vitro Diagnostic Industry in China - Blood Typing Instruments and Reagents III

Automatic blood grouping analyzer is an analyzer that can forward and reverse ABO blood group, Rh (D) blood group test, irregular antibody screening and cross-matching of blood test. It mainly consists of liquid handling system, incubator, centrifuge, interpretoscope, and microcomputer system.  It realizes distribution and transfer of samples and reagents by liquid handling system, conducts incubation of microcolumn gel card by incubator, carries out centrifugation of microcolumn gel card by centrifuge, finishes digital imaging of microcolumn gel card by interpretoscope, and completes comparative analysis of images of the microcolumn gel card by microcomputer, which allow it to achieve full automation and intellectualization of blood typing, irregular antibody screening, and cross-matching of blood test.

1. Automatic blood grouping analyzer based on MGT: MGT is the combined product of gel filtration technology and immunoreaction, and gap of gel plays the role of molecular sieve that only allows free erythrocytes to pass. Upon centrifugation by centrifuge, if erythrocytes without agglutination in micro-column gel are deposited at the bottom of agglutinatior, then it indicates negative reaction. It shall be deemed as agglutination if erythrocytes are agglutinated at upper or middle part of agglutinatior, which indicates positive reaction. The instrument is used in combination with suitable microcolumn gel card for automatic completion of the whole process of scanning, sample and reagent distribution, blood grouping cards handling, puncture, pipetting, incubation, centrifugation, image acquisition, and automatic interpretation.

2. Automatic blood grouping analyzer based on microplate technique: take microplate or a transparent 96-well plate as carrier, it shall be deemed as non-agglutination if erythrocytes are laid on bottom of micro-pores, and it shall be deemed as agglutination if erythrocytes deposit to the bottom of micro-pores and form concentrated dots. Microplate can be divided into two types of trapezoidal microplate and 96-well U-shaped microplate. Trapezoidal microplate uses Japan-based Olympus’s trapezoidal microplate for antigen-antibody immunoreaction, and uses core components of digital camera and digital camcorder (CCD) imaging technique for judgment of agglutination. U-shaped microplate uses microplate reader for scanning of transmittance within diameter of U-shaped pores, and then calculates relative transmittance by microplate reader software to get blood typing results.

3. Tube method-based automatic blood grouping analyzer: in recent years, domestic enterprises take blank reaction card (6-well card) as test carrier for blood cell antigen and antibody agglutination test pursuant to test principles and operations of classic blood group and serum test method of manual test tube method, test agglutination strength through changes in curve of transmitted flux, and automatically interpret test results. Such technique further enriched blood typing instruments, and provided users with new use experiences.

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